(no subject)

[beige_alert]

Last night I dreamed that I was in some sort of mass spectrometry lab and was telling others how I got my start in protein analysis, doing protein sequencing by Edman Degradation and got into an explanation of how it works, the phenyl isothiocyanate reacting with the N-terminal amino group, cleaving off the modified amino acid with trifluroacetic acid, which leaves a fresh N-terminal amine for the next cycle and the freed derivatized amino acid is then sent to an HLPC where they separate under pretty normal reverse phase conditions and show up on UV detection.

I haven't done this in a long time now, but a girl never forgets her first protein analysis method!

Comments

[pnictogen_wing]

that sounds like a very detailed dream!

[beige_alert]

I woke up from it at the end of sleep in the morning, and seriously I remembered it as me really giving the real explanation!

[xx_margufr0g_xx]

and last night i dreamt about a giraffe eating my soup!!!!!!!

[beige_alert]

Now that's a more typical dream!